ABSTRACT
The work conditions of dental surgeons (DS), associated with low compliance to precautionary standards, often lead to the colonization and dissemination of infectious agents. To assess the epidemiological and microbiological aspects of nasal colonization by Gram-negative bacteria in DS while teaching (dentistry teachers). The data were collected by application of a questionnaire and a nasal swab. The biochemical identification, the susceptibility profile and the detection of ß-lactamases were carried out in Vitek 2 compact®. 41 (77.3%) DS participated in the study, nine of them (22.0%) presented nasal colonization by Enterobacteriaceae, the participants were predominantly men (27/65.9%), over 50 years of age (24/58.5%). All of them confirmed using procedure gloves and a surgical mask while attending patients, with frequent (95.5%) hand washing although a statistical difference was found regarding personal habits (p=0.03). Enterobacter aerogenes (60.0%) was the most prevalent species, followed by Citrobacter koseri (20.0%). The intrinsic production of AmpC ß-lactamase by E. aerogenesspecies, which is multiresistant to antimicrobials, was present in the nasal cavity of 14.6% of the DS. There were high levels of nasal colonization by Enterobacteriaceae in teaching DS (22.0%), 14.6% had been colonized by multiresistant microorganisms and the results were associated with inadequate personal habits.
Subject(s)
Humans , Occupational Risks , Dentistry , Gram-Negative Bacteria , Nasal CavityABSTRACT
Canetas odontológicas de alta-rotação são instrumentos de grande uso na prática clínica, com descontaminação polêmica, por apresentar complexo funcionamento. Estudo microbiológico, realizado no Serviço Odontológico da Universidade Federal de Goiás, teve como objetivo avaliar a efetividade do reprocessamento de canetas odontológicas de alta-rotação em autoclave gravitacional, seguindo um protocolo. Observados os aspectos éticos, os dados foram obtidos por meio de análise microbiológica, informações do prontuário e questionário aos cirurgiões-dentistas. Após atendimento, as canetas foram reprocessadas e encaminhadas ao laboratório para coleta microbiológica. Amostras foram coletadas de seis canetas, utilizadas em 60 pacientes diferentes, seguindo o protocolo de reprocessamento. Swabs umedecidos com solução salina foram friccionados nas paredes internas da cabeça da caneta, transferidos para tubos contendo caldo tioglicolato de sódio e incubados por sete dias a 37ï°C. As 60 amostras demonstraram ausência de crescimento microbiano, o que evidencia a efetividade da esterilização em autoclaves gravitacionais, seguindo um protocolo de reprocessamento...
High rotation pen are instruments used a lot on dentistry clinical practice over polemic decontamination because its complex functioning. Microbiologic study conducted in the Dental Clinic of the Federal University of Goias, which aimed to evaluate effectiveness-reprocessing protocol of high rotation pen in a gravity-autoclave based on a protocol. All ethical aspects were observed and the data were obtained by microbiological analysis, information from medical records and questionnaire to dentists. After its use the pens were reprocessed and sent to the laboratory for microbiological collection. Samples of six pens used in 60 different patients were collected based on the reprocessing protocol. Swabs moistened in saline solution were swabbed inside the head pen's walls and transferred to a sodium thioglycollate solution and incubated for seven days at 37ºC. The 60 samples showed no microbial growth, demonstrating the effectiveness of sterilization in gravitational autoclaves based on reprocessing protocol...
Piezas de mano de alta rotación son instrumentos muy utilizados en la práctica clínica, con descontaminación controversia, por presentar operación compleja. Estudio microbiológico, realizado en el Servicio de Odontología de la Universidad Federal de Goiás, tuvo como objetivo evaluar la eficacia del reprocesamiento de piezas de mano de alta velocidad en autoclave gravitacional, siguiendo un protocolo. Observados todos los aspectos éticos, los datos fueron, obtenidos por medio de análisis microbiológicos, informaciones del prontuario y cuestionario a los dentistas. Después del tratamiento, las piezas de mano de alta rotación fueron reprocesadas y enviadas al laboratorio para sus recolecciones microbiológicas. Fue recolectado muestras en seis piezas de mano de alta rotación usadas en 60 pacientes diferentes según el protocolo de reprocesamiento. Swabshumedecidos con solución salina fueron frotadas en las paredes internas de la cabeza de la pieza y transferidos para la solución de tioglicolato de sodio y incubados siete días a 37ï°C. Las 60 muestras no mostró el crecimiento microbiano, lo que demuestra la eficacia de la esterilización en autoclaves gravitacionales siguiendo el protocolo de reprocesamiento...